Abstract


THE ROLE OF PATHOGENS, PROBIOTICS, AND THE BACTERIAL MICROFLORA IN ACUTE MONTIPORA WHITE SYNDROME

Tissue loss diseases destroy coral and can lead to colony mortality, which exacerbates the deteriorating state of the world's reefs. To defend against pathogens, the bacteria colonizing the coral, termed the normal microflora, are thought to protect the coral host through competition for nutrients and space or the production of antibacterial compounds. The bacterial pathogen V. coralliilyticus strain OCN008 infects and causes tissue loss in the reef-building coral Montipora capitata and produces the antibiotic andrimid. Under laboratory conditions, 85% of M. capitata fragments exposed to OCN008 developed tissue loss, however, a mutant strain incapable of producing andrimid displayed a 60% reduction in infection rate. Treatment of M. capitata fragments with antibiotics that cleared the microflora allowed the mutant strain to infect at wild-type levels, implicating andrimid in mitigation of the protective effects of the coral microflora. Strains of Pseudoalteromonas luteoviolacea and P. rubra isolated from healthy M. capitata release compound(s) into culture medium that inhibit OCN008 growth. When M. capitata fragments were pre-inoculated with P. luteoviolacea or P. rubra, OCN008 infection was reduced by 60% and 100%, respectively. Antibiotic production by OCN008 or its proximal effect, alteration of the coral microflora, is required for optimal infection and demonstration of a probiotic effect from bacteria of the coral microflora provides in vivo evidence for competitive antibacterial production between pathogens and the normal microflora.

Authors

Ushijima, B., University of Hawaii, USA, bushijima87@gmail.com

Videau, P., Oregon State University, USA, videaup@oregonstate.edu

Beurmann, S., University of Hawaii, USA, silviab@hawaii.edu

Poscablo, D., San Francisco State University , USA, donnaposcablo@gmail.com

Runyon, C., University of Hawaii, USA, runyon@hawaii.edu

Salcedo, M., University of Hawaii, USA, mayds@hawaii.edu

Vine, V., Rocky Mountain Libraries, USA, vcvine@hawaii.edu

Hemscheidt, H. K., University of Hawaii, USA, hemschei@hawaii.edu

Aeby, G. S., Hawaii Institute of Marine Biology, USA, greta@hawaii.edu

Callahan, S. M., University of Hawaii, USA, scallaha@hawaii.edu

Details

Oral presentation

Session #:13
Date: 06/23/2016
Time: 14:00
Location: 313 C

Presentation is given by student: Yes