Black Band Disease (BBD) is the first reported coral disease and the most widely distributed polymicrobial disease of corals. In BBD, a polymicrobial disease consortium dominated by the filamentous cyanobacterium Roseofilum reptotaenium displaces members of the epibiotic microbiome. We investigated the natural products chemistry and chemical ecology of BBD and nonaxenic cultures of R. reptotaenium. Within the BBD consortium, we identified lyngbic acid, a cyanobacterial secondary metabolite. It strongly inhibited quorum sensing (QS) in the Vibrio harveyi QS reporters, which depend on the presence of the CAI-1 receptor CqsS. Lyngbic acid inhibited luminescence in native coral Vibrio spp. that also possess CAI-1-mediated QS. R. reptotaenium also produces a novel malyngamide. Since malyngamides from other cyanobacteria are known to inhibit QS activity we also examined the functions of this new compound. The effects of these naturally occurring QS inhibitors on bacterial regulatory networks potentially contribute to the structuring of interactions within BBD consortia.


Paul, V. J., Smithsonian Institution, USA, paul@si.edu

Gunasekera, S. P., Smithsonian Institution, USA, gunasekeras@si.edu

Meyer, J. L., University of Florida, USA, juliemeyer@ufl.edu

Houk, L. J., Smithsonian Institution, USA, houkl@si.edu

Scott, R. M., University of Florida, rscott2367@ufl.edu

Teplitski, M., University of Florida, USA, maxtep@ufl.edu


Oral presentation

Session #:13
Date: 06/23/2016
Time: 15:00
Location: 313 C

Presentation is given by student: No